The Big Slough Preserve is just east of the Carlton Reserve. From the Carlton Reserve parking area follow the South Powerline to the east for approximately 7 miles to the fence line. There is an open gate at the fence line. The Big Slough Preserve is also accessible from North Port via the pedestrian/bicycle/equestrian bridge at Myakkahatchee Creek Environmental Park.
There are 52 miles of doubletrack trails and 15+ miles of singletrack trails in the Big Slough Preserve.